TIE-1

• Description:

  Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF) -like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse) /-4 (human) . It is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2.

• Synonyms:

  TIE1; TIE; JTK14

• NCBI Gene ID:

  7075

• UniProt:

  P35590

• Accession Number:

  NP_005415

• Accession Number mRNA:

  NM_005424

• Chromosomal Location:

  1p34-p33

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Human TIE-1 extracellular domain

• Clone:

  (#12D9)

• Applications:

  WB, IHC

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Bioactivity:

  WB: 1:500-1000; IHC: 1:100-400

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1