TIE-1

• Description:

  TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF) -like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

• Synonyms:

  Tie1; TIE; tie-1; D430008P04Rik

• NCBI Gene ID:

  21846

• UniProt:

  Q06806

• Accession Number:

  NP_035717.2

• Accession Number mRNA:

  NM_011587.2

• Chromosomal Location:

  4 D2.1; 4 50.0 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse sTIE-1 (RT Cat# S01-M42)

• Clone:

  Rabbit IgG

• Applications:

  WB

• Purification Method:

  Protein A purified

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: use at 2-5 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Rabbit