TIE-1

• Description:

  TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF) -like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

• Synonyms:

  TIE1; TIE; JTK14

• NCBI Gene ID:

  7075

• UniProt:

  P35590

• Accession Number:

  NP_005415

• Accession Number mRNA:

  NM_005424

• Chromosomal Location:

  1p34-p33

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human soluble TIE-1

• Clone:

  (#8C9)

• Applications:

  ELISA, WB

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  ELISA: Use at 1-15 µg/ml; Western blotting: Use at 1-2 µg/ml

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Notes:

  The monoclonal antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments, Western blotting, FACS and cell sorting.

• Host or Source:

  Mouse

• Isotype:

  IgG1