TIE-2

• Description:

  Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF) -like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse) /-4 (human) . Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

• Synonyms:

  TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B

• NCBI Gene ID:

  7010

• UniProt:

  Q02763

• Accession Number:

  NP_000450.2

• Accession Number mRNA:

  NM_000459.3

• Chromosomal Location:

  9p21

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Human soluble extracellular TIE-2

• Clone:

  (#tek2)

• Applications:

  ELISA, WB, FC

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  ELISA: Use at 1-2 µg/ml. Western blotting: Use at 2-5 µg/ml. FACS analysis: Use at 2-5 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Notes:

  The monoclonal antibody will detect native human TIE-2/tek in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments, Western blotting, FACS and cell sorting.

• Host or Source:

  Mouse

• Isotype:

  IgG1