Macroglobulin alpha 2

• Description :

  Human α2macroglobulin (hα2M) is a serum glycoprotein that has sequence similarity to other members of the α2M family including complement components C3, C4 and C5. α2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) . The mature protein is a tetramer (720 kDa) of 4 identicalsubunits (180 kDa), which form two disulfide bondlinked-dimers. As a general and irreversible protease inhibitor implicated in many processes, α2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of hα2M (residues 690-728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in α2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.

• Synonyms :

  A2M; A2MD; CPAMD5; FWP007; S863-7

• NCBI Gene ID :

  2

• UniProt :

  P01023

• Accession Number :

  NP_000005.2

• Accession Number mRNA :

  NM_000014.4

• Chromosomal Location :

  12p13.31

• Reactivity :

  Anti-Human

• Cross Reactivity :

  Human

• Target Antigen :

  Human recombinant alpha-2 macroglobulin

• Clone :

  (#4H44)

• Applications :

  WB

• Purification Method :

  Protein G chromatography

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  PBS

• Storage Conditions :

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source :

  Mouse

• Isotype :

  IgG1