Leptin, MTS-tagged

• Description:

  Recombinant ovine MTS-leptin, one polypeptide chain containing 157 amino and (extra 10 amino acids of the membrane translocating sequence Val-Leu-Leu-Pro-Val-Leu-Leu-Ala-Ala-Pro) derived from Kaposi virus and additional Ala at N-terminus acids . The resulting molecular mass of is ~ 17.5 kDa, MTS-ovine leptin was purified by proprietary chromatographic techniques.

• Synonyms:

  Lep; ob; obese

• NCBI Gene ID:

  443534

• UniProt:

  G8BLB2

• Accession Number:

  XP_027824581.2

• Accession Number mRNA:

  XM_027968780.2

• Reactivity:

  Ovine

• Cross Reactivity:

  Ovine

• Sequence:

  AAVLLPVLLAAPVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGLDFIPGLHPLLSLSKMDQTLAIYQQILASLPSRNVIQISNDLENLRDLLHLLAASKSCPLPQVRALESLESLGVVLEASLYSTEVVALSRLQGSLQDMLRQLDLSPG

• Assay Protocol:

  It is recommended to reconstitute the lyophilized MTS-ovine recombinant leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Bioactivity:

  MTS-ovine recombinant leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. In vivo experiments has shown potency equal but not higher that non tagged ovine leptin (A. Gertler, unpublished data) .

• Length:

  157

• Form:

  Lyophilized

• Reconstitution:

  0.4% NaHCO3, pH 8-9

• Molecular Weight:

  17.5 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  AAVLLP