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Leptin

CAT: 0209-500-062Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0209-500-062Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Recombinant ovine leptin was purified by proprietary chromatographic techniques, see Gertler et al. FEBS Lett. 442, 137-140 (1998) .
Synonyms
Lep; ob; obese
NCBI Gene ID
443534
UniProt
G8BLB2
Accession Number
XP_027824581.2
Accession Number mRNA
XM_027968780.2
Reactivity
Ovine
Cross Reactivity
Ovine
Sequence
AVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGLDFIPGLHPLLSLSKMDQTLAIYQQILASLPSRNVIQISNDLENLRDLLHLLAASKSCPLPQVRALESLESLGVVLEASLYSTEVVALSRLQGSLQDMLRQLDLSPG
Assay Protocol
It is recommended to reconstitute the lyophilized recombinant ovine leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 95.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
Recombinant ovine leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Length
146
Form
Lyophilized
Reconstitution
0.4% NaHCO3, pH 8-9
Molecular Weight
16.0 kDa
Host or Source
E. coli
N Terminal Sequence
AVPIR