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Leptin Recombinant Protein

CAT: 0209-500-058Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0209-500-058Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant chicken leptin is an one polypeptide chain containing 145 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Chicken leptin was prepared according to the sequence published by the groups of Taouis and McMutry, purified by proprietary chromatographic techniques, see Raver et al. Protein Expr Purif. 1998 Dec; 14(3):403-8.
CAS Number
9000-83-3
Synonyms
Lep; ob; obese
NCBI Gene ID
373955
UniProt
O42164
Accession Number
APC23099.1
Accession Number mRNA
KT970642.1
Host
E. coli
Origin Species
Chicken
Species Reactivity
Chicken
Sequence
AVPCQIFQDDTKTLIKTIVTRINDISHTSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDISPEC
Detection Range
Recombinant chicken leptin is biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity is however 5-10 fold lower as compared to mammalian leptins.
Endotoxin
< 0.1 ng/µg of protein (<1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length
146
Form
Lyophilized
Reconstitution
It is recommended to reconstitute the lyophilized recombinant chicken leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Molecular Weight
16.0 kDa
Shipping Conditions
Room Temperature
N Terminal Sequence
AVPCQ

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