Leptin A Recombinant Protein

CAT: 0209-500-074Size: 500 µgDry Ice: NoHazardous: No
CAT#:0209-500-074Size:500 µg
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Description
Full‐length cDNA encoding two leptin sequences (tLepA and tLepB) were identified in tilapia (Oreochromis niloticus). The cDNAs of tLepA and tLepB were 486 bp and 459 bp in length, encoding proteins of 161 aa and 152 aa, respectively. The tLepA‐ ‐expressing plasmid was transformed into E. coli and expressed as recombinant protein upon induction with nalidixic acid, found almost entirely in insoluble inclusion bodies (IBs). The protein was solubilized, refolded and purified to homogeneity by anion‐exchange chromatography. More information can be found in Shpilman et al. (2014) General and Comparative Endocrinology 270, 74-85.
CAS Number
9000-83-3
Synonyms
Lep; ob; obese
NCBI Gene ID
100704227
UniProt
I3KCE8
Accession Number
NP_001287979.1
Accession Number mRNA
NM_001301050.1
Host
E. coli
Origin Species
Tilapia
Species Reactivity
Tilapia
Sequence
APLPVEVVTMKSKVKWMAEQLVVRLDKDVQVPVNWTLNPPADDLDGTSSIETVLNGYNSLIPDTFKGVSQIKYDISSLTGYIHLWRQGHCSEQRPKPEVPGPLQELQSHKEFIQTVGIEALMRVKEFLNLLLKNLDQLETC
Detection Range
Monomeric and dimeric Tilapia leptins were biologically active in promoting proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor (hLepR), but their activity was four orders of magnitude lower than that of mammalian leptin. Furthermore, the Tilapia leptins were biologically active in promoting STAT‐LUC activation in COS7 cells transfected with Tilapia leptin receptor but not in cells transfected with human leptin receptor. Tilapia Leptin A was more active than Tilapia Leptin B.
Endotoxin
< 0.1 ng/µg of protein (<1EU/µg)
Purity
> 95.0% as determined by Gel filtration and SDS-PAGE gel.
Length
141
Form
Lyophilized
Reconstitution
It is recommended to reconstitute the lyophilized recombinant Tilapia leptin A in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
Molecular Weight
15.9 kDa
Shipping Conditions
Room Temperature
N Terminal Sequence
APLPVE

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