Leptin binding protein Recombinant Protein

• Description:

  Recombinant human leptin binding domain (hLBD), is one polypeptide chain containing 208 amino acids and an additional Ala at N-terminus acids. It consists of the cytokine binding domain of leptin receptor (amino acids 428-635 of human leptin receptor and having a molecular mass of ~ 24.5 kDa, It was purified by proprietary chromatographic techniques (see Sandowski et al. J. Biol. Chem. (2002) 277(48):46304-9.

• Synonyms:

  Lep; ob; obese

• NCBI Gene ID:

  3953

• UniProt:

  P48357

• Accession Number:

  NP_001003679.1

• Accession Number mRNA:

  NM_001003679.3

• Host:

  E. coli

• Origin Species:

  Human

• Species Reactivity:

  Human

• Sequence:

  AIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMD

• Detection Range:

  Recombinant human LBDL is fully biologically active as evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Length:

  208

• Form:

  Lyophilized

• Reconstitution:

  It is recommended to reconstitute the lyophilized hLBD in sterile water adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

• Molecular Weight:

  24.5 kDa

• Shipping Conditions:

  Room Temperature

• N Terminal Sequence:

  AIDVNI

• CAS Number:

  9000-83-3