IL-5R alpha EC

• Description :

  Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology. Mature human IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four-cysteine motif, a 20 aa transmembrane segment, and a 58 aa cytoplasmic domain. Within the ECD, human IL-5 R alpha shares 71% aa sequence identity with mouse and rat IL‑5 R alpha. Alternate splicing of human IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists. The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common beta chain (beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF. IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signalling‑competent receptor complex.

• Synonyms :

  Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA, CDw125, CD125

• NCBI Gene ID :

  3568

• UniProt :

  Q01344

• Accession Number :

  NP_000555.2

• Accession Number mRNA :

  NM_000564.4

• Reactivity :

  Anti-Human

• Cross Reactivity :

  Human

• Target Antigen :

  Human recombinant IL-5RalphaEC domain

• Clone :

  (#6J30)

• Applications :

  WB

• Purification Method :

  Protein G chromatography

• Assay Protocol :

  Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  PBS

• Storage Conditions :

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

• Host or Source :

  Mouse

• Isotype :

  IgG1