IFN gamma

• Description :

  Interferon-gamma (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Recombinant Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.

• Synonyms :

  IFNG; IFG; IFI

• NCBI Gene ID :

  3458

• UniProt :

  P01579

• Accession Number :

  NP_000610.2

• Accession Number mRNA :

  NM_000619

• Chromosomal Location :

  12q14

• Reactivity :

  Human

• Cross Reactivity :

  Monkey, Human, Mouse, Leech, Pig

• Sequence :

  MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

• Assay Protocol :

  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to ‑80°C.

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity :

  Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml (> 2 x 107 units/mg) .

• Length :

  144

• Form :

  Lyophilized

• Buffer :

  10mM Sodium Phosphate, pH 7.4

• Reconstitution :

  Water

• Molecular Weight :

  16.8 kDa

• Storage Conditions :

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.

• Host or Source :

  E. coli