Interferon gamma (IFN) R1

• Description:

  The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha) and IFN-gamma R2 (IFN-gamma R beta) . Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signalling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.

• Synonyms:

  Interferon gamma receptor 1, IFN-gamma receptor 1; IFN-gamma-R1, CDw119, Interferon gamma receptor alpha-chain (IFN-gamma-R-alpha), CD119

• NCBI Gene ID:

  3459

• UniProt:

  P15260

• Accession Number:

  NP_000407.1

• Accession Number mRNA:

  NM_000416.2

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Human recombinant IFN gamma RI EC domain

• Clone:

  (#7K51)

• Applications:

  WB, FC, N

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1