CCL-19

• Description:

  CCL19/MIP3β, also known as ELC (EBI1Ligand Chemokine), is a β chemokine that binds specifically to the chemokine receptor CCR7/EBI1/ BLR2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other β chemokines (20-30% aa sequence identity) . Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is downregulated by the antiinflammatory cytokine IL10. Recombinant CCL19 has been shown to be chemotactic for Tcells and Bcells. The CCL19 receptor (CCR7/ EBI1/ BLR2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly upregulated in Bcells infected with EpsteinBarr virus and T-cells infected with herpesvirus 6 or 7.

• Synonyms:

  Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3

• NCBI Gene ID:

  24047

• UniProt:

  O70460

• Accession Number:

  NP_036018

• Accession Number mRNA:

  NM_011888

• Chromosomal Location:

  4 A5; 4

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse CCL-19

• Clone:

  (#10J38)

• Applications:

  WB, N

• Purification Method:

  Protein G/A chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2