DPP4 ELISA Kit (Rat)

CAT: 0247-OKEH04388-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04388-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Dipeptidylpeptidase 4
Gene Aliases
Bile canaliculus domain-specific membrane glycoprotein; CD26; dipeptidyl peptidase 4; dipeptidyl peptidase IV; DPP IV; DPPIV; GP110 glycoprotein; T-cell activation antigen CD26.
Gene ID
25253
Accession Number
NP_036921.1
Reactivity
Rat, Rattus norvegicus
Target
Dipeptidyl peptidase 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Dpp4 ELISA Kit (Rat) (OKEH04388) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dpp4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Dpp4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dpp4 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Sensitivity
0.082 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
88 KDa
Specificity
Natural and recombinant Rat Dipeptidyl peptidase 4
Protein Length
767
NCBI Gene Symbol
Dpp4
Protein Name
P14740
Gene Name URL
Dpp4
Nucleotide Accession Number
NM_012789.1

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