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CD26/DPP4 ELISA Kit (Mouse)

CAT: 0247-OKBB00421-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00421-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 773 1540 2892 846 1685 3195 SD 39.4 104.72 216.9 54.99 124.69 274.77 CV% 5.1 6.8 7.5 6.5 7.4 8.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 773 1540 2892 846 1685 3195 Mean (pg/mL) 773 1540 2892 846 1685 3195 SD 39.4 104.72 216.9 54.99 124.69 274.77 SD 39.4 104.72 216.9 54.99 124.69 274.77 CV% 5.1 6.8 7.5 6.5 7.4 8.6 CV% 5.1 6.8 7.5 6.5 7.4 8.6
CAS Number
7732-18-5
Gene Name
Dipeptidyl-peptidase 4
Gene Aliases
ADABP, ADCP-2, ADCP2, Adenosine deaminase complexing protein 2, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV, Dipeptidyl peptidase IV membrane form, Dipeptidyl peptidase IV soluble form, Dipeptidylpeptidase 4, DPP 4, DPP IV, DPP4, DPP4_HUMAN, DPPIV, T-cell activation antigen CD26, TP103
Gene ID
13482
Accession Number
NP_001153015.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: S29-H760
Target
Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. glucoseBy fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse CD26 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD26 has been precoated onto 96-well plates. Standards (NS0, S29-H760) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD26 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD26 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse CD26
NCBI Gene Symbol
DPP4
Protein Name
Dipeptidyl peptidase 4
Gene Name URL
DPP4
Nucleotide Accession Number
NM_001159543.1

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