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DPP4 ELISA Kit (Human)

CAT: 0247-OKEH02728-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02728-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Dipeptidyl peptidase 4
Gene Aliases
ADABP; ADCP2; ADCP-2; adenosine deaminase complexing protein 2; CD26; dipeptidyl peptidase 4; dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) ; DPP IV; DPPIV; Gly-Pro naphthylamidase; Post-proline dipeptidyl aminopeptidase IV; T-cell activation antigen CD26; TP103; Xaa-Pro-dipeptidylaminopeptidase.
Gene ID
1803
Accession Number
NP_001926.2
Reactivity
Homo sapiens, Human
Target
Dipeptidyl peptidase 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DPP4 ELISA Kit (Human) (OKEH02728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPP4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for DPP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPP4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
62.5-4000ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
30.5 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
88 kDa
Specificity
Natural and recombinant Human Dipeptidyl peptidase 4
Protein Length
766
NCBI Gene Symbol
DPP4
Protein Name
P27487
Gene Name URL
DPP4
Nucleotide Accession Number
NM_001935.3

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