DPP4 ELISA Kit (Human)

CAT: 0247-OKBB00783-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00783-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 3074 9263 536 3241 9182 Standard deviation 26.55 196.73 574.3 26.26 269 661.1 CV (%) 4.5 6.4 6.2 4.9 8.3 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 590 3074 9263 536 3241 9182 Mean (pg/ml) 590 3074 9263 536 3241 9182 Standard deviation 26.55 196.73 574.3 26.26 269 661.1 Standard deviation 26.55 196.73 574.3 26.26 269 661.1 CV (%) 4.5 6.4 6.2 4.9 8.3 7.2 CV (%) 4.5 6.4 6.2 4.9 8.3 7.2
CAS Number
7732-18-5
Gene Name
Dipeptidyl-peptidase 4
Gene Aliases
ADABP, ADCP-2, ADCP2, Adenosine deaminase complexing protein 2, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV, Dipeptidyl peptidase IV membrane form, Dipeptidyl peptidase IV soluble form, Dipeptidylpeptidase 4, DPP 4, DPP IV, DPP4, DPP4_HUMAN, DPPIV, T-cell activation antigen CD26, TP103
Gene ID
1803
Accession Number
NP_001926.2
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: D34-P766
Target
Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human CD26 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD26 has been precoated onto 96-well plates. Standards (NS0, D34-P766) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD26 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CD26 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human CD26
NCBI Gene Symbol
DPP4
Protein Name
Dipeptidyl peptidase 4
Gene Name URL
DPP4
Nucleotide Accession Number
NM_001935.3

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