HAMP High Sensitivity ELISA Kit (Rat)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Hepcidin (Hepc) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Hepcidin antimicrobial peptide

• Gene Aliases:

  Hepc; hepcidin.

• Gene ID:

  84604

• Accession Number:

  NP_445921.1

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. May also have antimicrobial activity (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Hepcidin (Hepc) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Hepcidin (Hepc) and unlabeled High Sensitive Hepcidin (Hepc) (Standards or samples) with the pre-coated antibody specific to High Sensitive Hepcidin (Hepc) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Hepcidin (Hepc) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Hepcidin (Hepc) in the sample.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  246.9-20000pg/mL

• Sensitivity:

  < 87.3pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount HAMP Microplate 96 Wells (12 x 8 Well strips) HAMP Lyophilized Standard 2 100X HAMP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount HAMP Microplate 96 Wells (12 x 8 Well strips) HAMP Microplate 96 Wells (12 x 8 Well strips) HAMP Lyophilized Standard 2 HAMP Lyophilized Standard 2 100X HAMP HRP-Detector Anitbody 1 x 120 uL 100X HAMP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL

• NCBI Gene Symbol:

  Hamp

• Protein Name:

  Hepcidin

• Gene Name URL:

  Hamp

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_053469.1