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NEFL High Sensitivity ELISA Kit (Human)

CAT: 0247-OKCD01380-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01380-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Neurofilament light chain
Gene Aliases
68 kDa neurofilament protein; CMT1F; CMT2E; CMTDIG; light molecular weight neurofilament protein; neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; NF68; NFL; NF-L; PPP1R110; protein phosphatase 1, regulatory subunit 110.
Gene ID
4747
Accession Number
NP_006149.2
Reactivity
Homo sapiens|Human
Target
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Neurofilament, Light Polypeptide (NEFL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, cerebrospinal fluid and other biological fluids
Detection Range
1.56-100pg/mL
Recovery
Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 78-90 86 _x005F EDTA Plasma (n=5) 92-105 101 _x005F Heparin Plasma (n=5) 89-97 94 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-90 86 Serum (n=5) 78-90 86 EDTA Plasma (n=5) 92-105 101 EDTA Plasma (n=5) 92-105 101 Heparin Plasma (n=5) 89-97 94 Heparin Plasma (n=5) 89-97 94
Sensitivity
< 0.61 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-NEFL Microplate 96 Wells (12 x 8 Well Strips) _x005F NEFL Lyophilized Standard 2 _x005F 100X Biotinylated NEFL Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-NEFL Microplate 96 Wells (12 x 8 Well Strips) Anti-NEFL Microplate 96 Wells (12 x 8 Well Strips) NEFL Lyophilized Standard 2 NEFL Lyophilized Standard 2 100X Biotinylated NEFL Detector Antibody 120 uL 100X Biotinylated NEFL Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL) . No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.
NCBI Gene Symbol
NEFL
Protein Name
Neurofilament light polypeptide
Gene Name URL
NEFL
Nucleotide Accession Number
NM_006158.4

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