OVAL High Sensitivity ELISA Kit

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Ovalbumin (OVA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Ovalbumin (OVA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  SERPINB14

• Gene Aliases :

  OVA, SERPINB14

• Gene ID :

  396058

• Accession Number :

  NP_990483.1

• Reactivity :

  All Species

• Target :

  Non-inhibitory serpin. Storage protein of egg white.

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Ovalbumin (OVA) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Ovalbumin (OVA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Ovalbumin (OVA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Ovalbumin (OVA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  78-5,000pg/mL

• Recovery :

  Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 92-101 95 _x005F EDTA Plasma (n=5) 92-105 96 _x005F Heparin Plasma (n=5) 79-89 84 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 92-101 95 Serum (n=5) 92-101 95 EDTA Plasma (n=5) 92-105 96 EDTA Plasma (n=5) 92-105 96 Heparin Plasma (n=5) 79-89 84 Heparin Plasma (n=5) 79-89 84

• Sensitivity :

  <28 pg/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount _x005F Anti-OVAL Microplate 96 Wells (12 x 8 Well Strips) _x005F OVAL Lyophilized Standard 2 _x005F 100X Biotinylated OVAL Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-OVAL Microplate 96 Wells (12 x 8 Well Strips) Anti-OVAL Microplate 96 Wells (12 x 8 Well Strips) OVAL Lyophilized Standard 2 OVAL Lyophilized Standard 2 100X Biotinylated OVAL Detector Antibody 120 uL 100X Biotinylated OVAL Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Ovalbumin (OVA) . No significant cross-reactivity or interference between Ovalbumin (OVA) and analogues was observed.

• NCBI Gene Symbol :

  OVAL

• Protein Name :

  Ovalbumin

• Gene Name URL :

  OVAL

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_205152.2