Apoa4 High Sensitivity ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Apolipoprotein A4 (APOA4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Apolipoprotein A4 (APOA4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Apolipoprotein A-IV

• Gene Aliases:

  Ap; Apoa-4; apoA-IV; apo-AIV; apolipoprotein A4; apolipoprotein A-IV.

• Gene ID:

  11808

• Accession Number:

  NP_031494.2

• Reactivity:

  Mouse|Mus musculus

• Target:

  May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Apolipoprotein A4 (APOA4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Apolipoprotein A4 (APOA4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Apolipoprotein A4 (APOA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Apolipoprotein A4 (APOA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  7.8-500pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 88-104 97 EDTA Plasma (n=5) 88-99 94 Heparin Plasma (n=5) 78-92 87 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 88-104 97 Serum (n=5) 88-104 97 EDTA Plasma (n=5) 88-99 94 EDTA Plasma (n=5) 88-99 94 Heparin Plasma (n=5) 78-92 87 Heparin Plasma (n=5) 78-92 87

• Sensitivity:

  2.7 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-Apoa4 Microplate 96 Wells (12 x 8 Well Strips) Apoa4 Lyophilized Standard 2 100X Biotinylated Apoa4 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Apoa4 Microplate 96 Wells (12 x 8 Well Strips) Anti-Apoa4 Microplate 96 Wells (12 x 8 Well Strips) Apoa4 Lyophilized Standard 2 Apoa4 Lyophilized Standard 2 100X Biotinylated Apoa4 Detector Antibody 120 uL 100X Biotinylated Apoa4 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4) . No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.

• NCBI Gene Symbol:

  Apoa4

• Protein Name:

  Apolipoprotein A-IV

• Gene Name URL:

  Apoa4

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_007468.2