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IL8 High Sensitivity ELISA Kit (Human)

CAT: 0247-OKCD01311-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01311-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Interleukin 8 (IL8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
C-X-C motif chemokine ligand 8
Gene Aliases
Alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; emoctakin; GCP1; GCP-1; granulocyte chemotactic protein 1; IL8; interleukin 8; interleukin-8; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; LYNAP; MDNCF; MONAP; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP1; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; SCYB8; small inducible cytokine subfamily B, member 8; T-cell chemotactic factor; tumor necrosis factor-induced gene 1.
Gene ID
3576
Accession Number
NP_000575.1
Reactivity
Homo sapiens|Human
Target
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 8 (IL8) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 8 (IL8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
3.9-250pg/mL
Recovery
Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 93-101 97 _x005F EDTA Plasma (n=5) 90-101 98 _x005F Heparin Plasma (n=5) 89-104 93 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93-101 97 Serum (n=5) 93-101 97 EDTA Plasma (n=5) 90-101 98 EDTA Plasma (n=5) 90-101 98 Heparin Plasma (n=5) 89-104 93 Heparin Plasma (n=5) 89-104 93
Sensitivity
1.7 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-IL8 Microplate 96 Wells (12 x 8 Well Strips) _x005F IL8 Lyophilized Standard 2 _x005F 100X Biotinylated IL8 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-IL8 Microplate 96 Wells (12 x 8 Well Strips) Anti-IL8 Microplate 96 Wells (12 x 8 Well Strips) IL8 Lyophilized Standard 2 IL8 Lyophilized Standard 2 100X Biotinylated IL8 Detector Antibody 120 uL 100X Biotinylated IL8 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8) . No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.
NCBI Gene Symbol
CXCL8
Protein Name
Interleukin-8
Gene Name URL
CXCL8
Nucleotide Accession Number
NM_000584.3

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