5-Hydroxytryptophan ELISA Kit

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level 5-Hydroxytryptophan (5-HTP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level 5-Hydroxytryptophan (5-HTP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

5-hydroxy-L-tryptophan; L-5-Hydroxytryptophan; Oxitriptan; 4350-09-8; 5-hydroxytryptophan

All Species

5-Hydroxytryptophan is an aromatic amino acid with antidepressant activity. In vivo, oxitriptan (or 5-hydroxytryptophan) is converted into 5-hydroxytryptamine (5-HT or serotonin) as well as other neurotransmitters. Oxitriptan may exert its antidepressant activity via conversion to serotonin or directly by binding to serotonin (5-HT) receptors within the central nervous system (CNS) . Endogenous oxitriptan is produced from the essential amino acid L-tryptophan. The exogenous therapeutic form is isolated from the seeds of the African plant Griffonia simplicifolia.

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Colorimetric

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxytryptophan (5-HTP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptophan (5-HTP) and unlabeled 5-Hydroxytryptophan (5-HTP) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptophan (5-HTP) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxytryptophan (5-HTP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxytryptophan (5-HTP) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma and other biological fluids.

4.93-400ng/mL

Matrix Recovery Range (%) Average (%) serum (n=5) 94-105 98 EDTA plasma (n=5) 99-105 102 heparin plasma (n=5) 90-97 94 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 94-105 98 serum (n=5) 94-105 98 EDTA plasma (n=5) 99-105 102 EDTA plasma (n=5) 99-105 102 heparin plasma (n=5) 90-97 94 heparin plasma (n=5) 90-97 94

1.93 ng/mL

2°C to 8°C|-20°C

Component Amount Anti-5-Hydroxytryptophan Microplate 96 Wells (12 x 8 Well Strips) 5-Hydroxytryptophan Lyophilized Standard 2 100X 5-Hydroxytryptophan Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-5-Hydroxytryptophan Microplate 96 Wells (12 x 8 Well Strips) Anti-5-Hydroxytryptophan Microplate 96 Wells (12 x 8 Well Strips) 5-Hydroxytryptophan Lyophilized Standard 2 5-Hydroxytryptophan Lyophilized Standard 2 100X 5-Hydroxytryptophan Biotin Complex 120 uL 100X 5-Hydroxytryptophan Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

5-HTP

5-Hydroxytryptophan