Products for Research Use Only
RegisterLogin

IL-5 ELISA Kit (Human)

CAT: 0247-OKBB00187-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00187-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 44.6 168 338 43.8 188 329 St. Dev. 2.32 6.89 14.87 2.63 10.34 21.06 %CV 5.20 4.10 4.39 6.00 5.5 6.40 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 44.6 168 338 43.8 188 329 Mean (pg/mL) 44.6 168 338 43.8 188 329 St. Dev. 2.32 6.89 14.87 2.63 10.34 21.06 St. Dev. 2.32 6.89 14.87 2.63 10.34 21.06 %CV 5.20 4.10 4.39 6.00 5.5 6.40 %CV 5.20 4.10 4.39 6.00 5.5 6.40
CAS Number
7732-18-5
Gene Name
Interleukin 5
Gene Aliases
B cell differentiation factor I, B-cell differentiation factor I, Colony stimulating factor eosinophil, EDF, Eosinophil differentiation factor, IL 5, IL-5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor eosinophil), Interleukin-5, Interleukin5, T cell replacing factor, T-cell replacing factor, TRF
Gene ID
3567
Accession Number
NP_000870.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: I20-S134
Target
Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF) . IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation.1 The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators.2 IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3 Human and mouse of IL-5 share 70% amino acid sequence homology.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-5 has been precoated onto 96-well plates. Standards (NS0, I20-S134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-5 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA) and urine
Detection Range
7.8 pg/ml - 500 pg/ml
Sensitivity
<2 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human IL-5
NCBI Gene Symbol
IL5
Protein Name
Interleukin-5
Gene Name URL
IL5
Nucleotide Accession Number
NM_000879.2

Related Products

CatalogName
OKBB00188IL-5 ELISA Kit (Mouse) (OKBB00188)
OKRC00624-96WOKRC00624-96W - IL-5 R alpha ELISA Kit (Human)
OKDB00019-96WIL-5 ELISpot Assay Kit (Human)
OKDB00019-5X96WIL-5 ELISpot Assay Kit (Human)
OKRC00097-96WOKRC00097-96W - IL-5 ELISA Kit (Cat)
OKRC01228-96WOKRC01228-96W - IL-5 ELISA Kit (Mouse)
GWB-ZZD137-96WHuman IL-18 ELISA Kit
OKBB00184-96WIL-4 ELISA Kit (Human)
OKBB00380-96WIL-29 ELISA Kit (Human)
OKBB00568-96WIL-18R1 ELISA Kit (Human)

Alternative Products

CatalogName
MBS9135753-01Human IL-5 ELISA Kit
MBS335538-01Human IL-5 ELISA Set
KET6016-01ELISA kit for Human IL-5
3490-1HP-1ELISA Pro: Human IL-5
DY205-05Human IL-5 DuoSet ELISA
K4156-100IL-5 (Human) ELISA Kit
M850025048Human IL-5 ELISA Discovery

Popular Products