1,5-Anhydroglucitol ELISA Kit

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level 1,5-Anhydroglucitol (1,5-AG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level 1,5-Anhydroglucitol (1,5-AG) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Aliases:

  1 1,5-Anhydro-D-glucitol; 1,5-Anhydroglucitol; 154-58-5; 1,5-ANHYDROSORBITOL

• Reactivity:

  All Species

• Target:

  1, 5-Anhydrosorbitol or 1, 5-anhydroglucitol (1, 5-AG) is a validated marker of short-term glycemic control. This substance is derived mainly from food, is well absorbed in the intestine, and is distributed to all organs and tissues. It is metabolically stable, being excreted in the urine when its level exceeds the renal threshold. It is reabsorbed in the renal tubules and is competitively inhibited by glucosuria, which leads to a reduction in its level in serum. The correlation between this reduction and the amount of glucose present in urine is so close that 1, 5 AG can be used as a sensitive, day-to-day, real-time marker of glycemic control. It provides useful information on current glycemic control and is superior to both hemoglobin A1C and fructosamine in detecting near-normoglycemia. 1, 5-AG in human plasma has been proposed for several years as a short-term, retrospective marker of glycemic control and seems to be the most suitable parameter for monitoring glucose excursions. The decrease in serum 1, 5-AG is very sensitive to urinary glucose excretion. It is a metabolically inert polyol that competes with glucose for reabsorption in the kidneys. Otherwise stable levels of 1, 5-AG are rapidly depleted as blood glucose levels exceed the renal threshold for glucosuria. 1, 5-AG is also more tightly associated with glucose fluctuations and postprandial glucose.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Detection Method:

  Colorimetric

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1,5-Anhydroglucitol (1,5-AG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1,5-Anhydroglucitol (1,5-AG) and unlabeled 1,5-Anhydroglucitol (1,5-AG) (Standards or samples) with the pre-coated antibody specific to 1,5-Anhydroglucitol (1,5-AG) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids.

• Detection Range:

  1.9-150ug/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) _x005F serum (n=5) 92-105 97 _x005F EDTA plasma (n=5) 94-101 97 _x005F heparin plasma (n=5) 95-102 99 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 92-105 97 serum (n=5) 92-105 97 EDTA plasma (n=5) 94-101 97 EDTA plasma (n=5) 94-101 97 heparin plasma (n=5) 95-102 99 heparin plasma (n=5) 95-102 99

• Sensitivity:

  < 0.7 ug/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount _x005F Anti-1,5-Anhydroglucitol Microplate 96 Wells (12 x 8 Well Strips) _x005F 1,5-Anhydroglucitol Lyophilized Standard 2 _x005F 1,5-Anhydroglucitol Biotin Complex 1 _x005F 100X Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Biotin Complex Diluent 1 x 12 mL _x005F Avidin/HRP Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Biotin Complex Reconstitution Buffer 1 x 300 uL _x005F Component Amount Component Amount Anti-1,5-Anhydroglucitol Microplate 96 Wells (12 x 8 Well Strips) Anti-1,5-Anhydroglucitol Microplate 96 Wells (12 x 8 Well Strips) 1,5-Anhydroglucitol Lyophilized Standard 2 1,5-Anhydroglucitol Lyophilized Standard 2 1,5-Anhydroglucitol Biotin Complex 1 1,5-Anhydroglucitol Biotin Complex 1 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL Biotin Complex Reconstitution Buffer 1 x 300 uL Biotin Complex Reconstitution Buffer 1 x 300 uL

• Notes:

  Formerly GWB-L4IXT1

• NCBI Gene Symbol:

  1,5-AG

• Protein Name:

  1,5-Anhydroglucitol

• Gene Name URL:

  1,5-AG

• CAS Number:

  7732-18-5