REG3A ELISA Kit (Human)

CAT: 0247-OKBB01252-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB01252-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1176 6316 19450 1433 6401 19872 Standard deviation 58.80 385.28 836.35 65.92 409.66 1430.78 CV (%) 5.0 6.1 4.3 4.6 6.4 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1176 6316 19450 1433 6401 19872 Mean (pg/ml) 1176 6316 19450 1433 6401 19872 Standard deviation 58.80 385.28 836.35 65.92 409.66 1430.78 Standard deviation 58.80 385.28 836.35 65.92 409.66 1430.78 CV (%) 5.0 6.1 4.3 4.6 6.4 7.2 CV (%) 5.0 6.1 4.3 4.6 6.4 7.2
CAS Number
7732-18-5
Gene Name
Regenerating family member 3 alpha
Gene Aliases
Regenerating islet-derived protein 3-alpha; REG-3-alpha; Hepatointestinal pancreatic protein; HIP; PAP; Human proislet peptide; Pancreatitis-associated protein 1; Regenerating islet-derived protein III-alpha; Reg III-alpha; REG3A; PAP1
Gene ID
5068
Accession Number
NP_002571.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: E27-D175
Target
Regenerating islet-derived protein 3 alpha (or Regenerating islet-derived protein III-alpha) formerly known as HIP/PAP (Hepatocarcinoma-Intestine-Pancreas/Pancreatitis-Associated Protein) and peptide 23 is a protein that in humans is encoded by the REG3A gene. This gene is mapped to 2p12. It encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human REG3A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for REG3A has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: E27-D175) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for REG3A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<0.1 ng/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human REG3A
NCBI Gene Symbol
REG3A
Protein Name
Regenerating islet-derived protein 3-alpha
Gene Name URL
REG3A
Nucleotide Accession Number
NM_002580.2

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