REG3A ELISA Kit (Mouse)

CAT: 0247-OKCA02188-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA02188-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
AV063448
Gene ID
19694
Accession Number
NP_035389
Reactivity
Mouse
Target
Bactericidal C-type lectin.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Reg3a ELISA Kit (Mouse) (OKCA02188) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for REG3A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for REG3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample REG3A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 94 90-97 EDTA plasma (n=4) 88 84-93 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 94 90-97 Serum (n=5) 94 90-97 EDTA plasma (n=4) 88 84-93 EDTA plasma (n=4) 88 84-93
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
REG3A
Protein Name
Regenerating islet-derived protein 3-alpha
Gene Name URL
REG3A
Nucleotide Accession Number
NM_011259

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