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REG3A ELISA Kit (Mouse)

CAT: 0247-OKCD02840-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02840-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Regenerating islet-derived 3 alpha
Gene Aliases
AV063448; generating islet-derived, mouse homolog 3 alpha; islet of Langerhans regenerating protein 3; lithostathine 3; pancreatitis-associated protein 2; reg III-alpha; REG-3-alpha; regenerating islet-derived protein 3-alpha; regenerating islet-derived protein III-alpha; RegIII (alpha) .
Gene ID
19694
Accession Number
NP_035389.1
Reactivity
Mouse|Mus musculus
Target
Bactericidal C-type lectin (By similarity) . Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Alpha (REG3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regenerating Islet Derived Protein 3 Alpha (REG3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 90-104 98 EDTA Plasma (n=5) 80-102 85 Heparin Plasma (n=5) 96-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 90-104 98 Serum (n=5) 90-104 98 EDTA Plasma (n=5) 80-102 85 EDTA Plasma (n=5) 80-102 85 Heparin Plasma (n=5) 96-105 101 Heparin Plasma (n=5) 96-105 101
Sensitivity
6.7 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-REG3A Microplate 96 Wells (12 x 8 Well strips) REG3A Lyophilized Standard 2 100X Biotinylated REG3A Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-REG3A Microplate 96 Wells (12 x 8 Well strips) Anti-REG3A Microplate 96 Wells (12 x 8 Well strips) REG3A Lyophilized Standard 2 REG3A Lyophilized Standard 2 100X Biotinylated REG3A Detector Antibody 1 x 120 uL 100X Biotinylated REG3A Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Alpha (REG3a) . No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Alpha (REG3a) and analogues was observed.
Protein Length
175
NCBI Gene Symbol
Reg3a
Protein Name
Regenerating islet-derived protein 3-alpha
Gene Name URL
Reg3a
Nucleotide Accession Number
NM_011259.1

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