REG3A ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Regenerating islet-derived 3 alpha

• Gene Aliases:

  AV063448; generating islet-derived, mouse homolog 3 alpha; islet of Langerhans regenerating protein 3; lithostathine 3; pancreatitis-associated protein 2; reg III-alpha; REG-3-alpha; regenerating islet-derived protein 3-alpha; regenerating islet-derived protein III-alpha; RegIII (alpha) .

• Gene ID:

  19694

• Accession Number:

  NP_035389.1

• Reactivity:

  Mouse|Mus musculus

• Target:

  Bactericidal C-type lectin (By similarity) . Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Alpha (REG3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regenerating Islet Derived Protein 3 Alpha (REG3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  15.6-1000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 90-104 98 EDTA Plasma (n=5) 80-102 85 Heparin Plasma (n=5) 96-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 90-104 98 Serum (n=5) 90-104 98 EDTA Plasma (n=5) 80-102 85 EDTA Plasma (n=5) 80-102 85 Heparin Plasma (n=5) 96-105 101 Heparin Plasma (n=5) 96-105 101

• Sensitivity:

  6.7 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-REG3A Microplate 96 Wells (12 x 8 Well strips) REG3A Lyophilized Standard 2 100X Biotinylated REG3A Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-REG3A Microplate 96 Wells (12 x 8 Well strips) Anti-REG3A Microplate 96 Wells (12 x 8 Well strips) REG3A Lyophilized Standard 2 REG3A Lyophilized Standard 2 100X Biotinylated REG3A Detector Antibody 1 x 120 uL 100X Biotinylated REG3A Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Alpha (REG3a) . No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Alpha (REG3a) and analogues was observed.

• Protein Length:

  175

• NCBI Gene Symbol:

  Reg3a

• Protein Name:

  Regenerating islet-derived protein 3-alpha

• Gene Name URL:

  Reg3a

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_011259.1