Reg3a ELISA Kit (Mouse)

CAT: 0247-OKBB01253-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB01253-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 174 502 32 209 528 Standard deviation 2.19 9.05 23.59 2.05 11.70 23.23 CV (%) 7.3 5.2 4.7 6.4 5.6 4.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 174 502 32 209 528 Mean (pg/ml) 30 174 502 32 209 528 Standard deviation 2.19 9.05 23.59 2.05 11.70 23.23 Standard deviation 2.19 9.05 23.59 2.05 11.70 23.23 CV (%) 7.3 5.2 4.7 6.4 5.6 4.4 CV (%) 7.3 5.2 4.7 6.4 5.6 4.4
CAS Number
7732-18-5
Gene Name
Regenerating islet-derived 3 alpha
Gene Aliases
Regenerating islet-derived protein 3-alpha; REG-3-alpha; Islet of Langerhans regenerating protein 3; Lithostathine 3; Pancreatitis-associated protein 2; Regenerating islet-derived protein III-alpha; Reg III-alpha; Reg3a; Pap2
Gene ID
19694
Accession Number
NP_035389.1
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: E27-Q175
Target
Regenerating islet-derived protein 3 alpha is a protein that in humans is encoded by the REG3A gene. It is mapped to 6 C3; 6 34.76 cM. This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse REG3A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for REG3A has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: E27-Q175) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for REG3A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse REG3A
NCBI Gene Symbol
REG3A
Protein Name
Regenerating islet-derived protein 3-alpha
Gene Name URL
REG3A
Nucleotide Accession Number
NM_011259.1

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