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SERPING1 ELISA Kit (Human)

CAT: 0247-OKBB01169-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01169-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1297 6095 242 1418 6290 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 CV (%) 6.3 4.9 6 7.6 6.7 6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 1297 6095 242 1418 6290 Mean (pg/ml) 222 1297 6095 242 1418 6290 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 CV (%) 6.3 4.9 6 7.6 6.7 6 CV (%) 6.3 4.9 6 7.6 6.7 6
CAS Number
7732-18-5
Gene Name
Serpin family G member 1
Gene Aliases
Plasma protease C1 inhibitor; C1 Inh; C1Inh; C1 esterase inhibitor; C1-inhibiting factor; Serpin G1; SERPING1; C1IN; C1NH
Gene ID
710
Accession Number
NP_000053.2
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: N23-A500
Target
This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE) . Alternative splicing results in multiple transcript variants encoding the same isoform.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human Serpin G1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin G1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: N23-A500) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin G1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Serpin G1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human Serpin G1
NCBI Gene Symbol
SERPING1
Protein Name
Plasma protease C1 inhibitor
Gene Name URL
SERPING1
Nucleotide Accession Number
NM_000062.2

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