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SERPING1 ELISA Kit (Mouse)

CAT: 0247-OKCA02183-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02183-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
C1I, C1n, C1nh, C1INH
Gene ID
12258
Accession Number
NP_033906
Reactivity
Mouse
Target
Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Serping1 ELISA Kit (Mouse) (OKCA02183) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPING1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPING1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SERPING1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
5.6 ng/mL-360 ng/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 88 85-92 EDTA plasma (n=4) 96 93-99 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 88 85-92 Serum (n=5) 88 85-92 EDTA plasma (n=4) 96 93-99 EDTA plasma (n=4) 96 93-99
Sensitivity
1.4 ng/mL.
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SERPING1
Protein Name
Plasma protease C1 inhibitor
Gene Name URL
SERPING1
Nucleotide Accession Number
NM_009776

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