Serping1 ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Serine (or cysteine) peptidase inhibitor, clade G, member 1

• Gene Aliases:

  C1 esterase inhibitor; C1 Inh; C1 inhibitor; C1I; C1INH; C1-inhibiting factor; C1n; C1nh; complement component 1 inhibitor; plasma protease C1 inhibitor; serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1; serine (or cysteine) proteinase inhibitor, clade G, member 1; serpin G1.

• Gene ID:

  12258

• Accession Number:

  NP_033906.2

• Reactivity:

  Mouse|Mus musculus

• Target:

  Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement 1 Inhibitor (C1INH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement 1 Inhibitor (C1INH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  15.6-1,000ng/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) Serum (n=5) 96-103 101 EDTA Plasma (n=5) 78-96 83 Heparin Plasma (n=5) 84-92 88 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 96-103 101 Serum (n=5) 96-103 101 EDTA Plasma (n=5) 78-96 83 EDTA Plasma (n=5) 78-96 83 Heparin Plasma (n=5) 84-92 88 Heparin Plasma (n=5) 84-92 88

• Sensitivity:

  < 5.9 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-Serping1 Microplate 96 Wells (12 x 8 Well Strips) Serping1 Lyophilized Standard 2 x 1,000 ng 100X Biotinylated Serping1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Serping1 Microplate 96 Wells (12 x 8 Well Strips) Anti-Serping1 Microplate 96 Wells (12 x 8 Well Strips) Serping1 Lyophilized Standard 2 x 1,000 ng Serping1 Lyophilized Standard 2 x 1,000 ng 100X Biotinylated Serping1 Detector Antibody 120 uL 100X Biotinylated Serping1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBKB6

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Complement 1 Inhibitor (C1INH) . No significant cross-reactivity or interference between Complement 1 Inhibitor (C1INH) and analogues was observed.

• Protein Length:

  504

• NCBI Gene Symbol:

  Serping1

• Protein Name:

  Plasma protease C1 inhibitor

• Gene Name URL:

  Serping1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009776.3