Hemoglobin High Sensitivity Colorimetric Detection Kit

• Reproducibility:

  Sample Hemoglobin Conc. (ug/mL) %CV 1 11.05 2.8 2 7.45 2.9 3 4.26 2.0 Sample Hemoglobin Conc. (ug/mL) %CV Sample Hemoglobin Conc. (ug/mL) %CV 1 11.05 2.8 1 11.05 2.8 2 7.45 2.9 2 7.45 2.9 3 4.26 2.0 3 4.26 2.0 Sample Hemoglobin Conc. (ug/mL) %CV 1 9.24 8.7 2 6.22 9.5 3 3.77 9.9 Sample Hemoglobin Conc. (ug/mL) %CV Sample Hemoglobin Conc. (ug/mL) %CV 1 9.24 8.7 1 9.24 8.7 2 6.22 9.5 2 6.22 9.5 3 3.77 9.9 3 3.77 9.9

• Gene Name:

  Hemoglobin High Sensitivity Colorimetric

• Type:

  Application Kit

• Detection Method:

  Colorimetric

• Assay Principle:

  The DetectX® High Sensitivity Hemoglobin Detection Kit is designed to quantitatively measure all forms of hemoglobin present in plasma and serum. Please read the complete kit insert before performing this assay. A human hemoglobin standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate and the ready-to-use DetectX® Hemoglobin Detection Reagent is added to each well. The plate is incubated for 30 minutes at room temperature. Stop solution is added at the end of 30 minutes and the plate is read at 450 nm. The concentration of the hemoglobin in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum and Plasma

• Sensitivity:

  0.053 ug/mL

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Clear 96 Well Plates 2 or 2 by 5 Each Hemoglobin Standard 90 μL or 450 uL Assay Buffer Concentrate 28 mL or 55 mL DetectX® Hemoglobin Detection Reagent 11 mL or 55 mL Stop Solution 5 mL or 25 mL Component Quantity Component Quantity Clear 96 Well Plates 2 or 2 by 5 Each Clear 96 Well Plates 2 or 2 by 5 Each Hemoglobin Standard 90 μL or 450 uL Hemoglobin Standard 90 μL or 450 uL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL DetectX® Hemoglobin Detection Reagent 11 mL or 55 mL DetectX® Hemoglobin Detection Reagent 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL