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Glucose Colorimetric Detection Kit

CAT: 0247-OKAU00114-2PLATESize: 2 PlatesDry Ice: NoHazardous: No
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CAT#:0247-OKAU00114-2PLATESize:2 Plates
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Sample Glucose Conc. (mg/dL) %CV 1 13.96 4.1 2 9.54 3.4 3 1.78 10.5 Sample Glucose Conc. (mg/dL) %CV Sample Glucose Conc. (mg/dL) %CV 1 13.96 4.1 1 13.96 4.1 2 9.54 3.4 2 9.54 3.4 3 1.78 10.5 3 1.78 10.5 Sample Glucose Conc. (mg/dL) %CV 1 13.19 11.2 2 9.40 6.4 3 1.59 9.4 Sample Glucose Conc. (mg/dL) %CV Sample Glucose Conc. (mg/dL) %CV 1 13.19 11.2 1 13.19 11.2 2 9.40 6.4 2 9.40 6.4 3 1.59 9.4 3 1.59 9.4
Gene Name
Glucose Colorimetric
Type
Application Kit
Detection Method
Colorimetric
Assay Principle
The DetectX® Glucose Colorimetric Detection Kit is designed to quantitatively measure glucose in a variety of samples. Please read the complete kit insert before performing this assay. A b-D-glucose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Colorimetric Substrate and horseradish peroxidase and the reaction initiated by addition of glucose oxidase. The reaction is incubated at room temperature for 30 minutes. The glucose oxidase reacts with glucose to produce hydrogen peroxide which, in the presence of HRP, reacts with the Substrate to convert the colorless substrate into a pink-colored product. The pink product is read at 560 nm. Increasing levels of glucose cause a linear increase in color.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Buffers and TCM
Sensitivity
0.413 mg/dL
Reconstitution
2°C to 8°C
Components
Component Quantity Clear Half Area 96 Well Plates 2 Plates Glucose Standard 90 uL Assay Buffer 50 mL Substrate 5 mL Horseradish Peroxidase Concentrate 60 uL Glucose Oxidase Concentrate 600 uL Component Quantity Component Quantity Clear Half Area 96 Well Plates 2 Plates Clear Half Area 96 Well Plates 2 Plates Glucose Standard 90 uL Glucose Standard 90 uL Assay Buffer 50 mL Assay Buffer 50 mL Substrate 5 mL Substrate 5 mL Horseradish Peroxidase Concentrate 60 uL Horseradish Peroxidase Concentrate 60 uL Glucose Oxidase Concentrate 600 uL Glucose Oxidase Concentrate 600 uL