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Ctsg High Sensitivity ELISA Kit (Mouse)

CAT: 0247-OKCD01379-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01379-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Cathepsin G (CTSG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Cathepsin G (CTSG) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Cathepsin G
Gene Aliases
CatG; cathepsin G; vimentin-specific protease; VSP.
Gene ID
13035
Accession Number
NP_031826.1
Reactivity
Mouse|Mus musculus
Target
This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Cathepsin G (CTSG) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Cathepsin G (CTSG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Cathepsin G (CTSG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Cathepsin G (CTSG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
3.12-200pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 78-90 81 EDTA Plasma (n=5) 97-105 101 Heparin Plasma (n=5) 80-103 99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-90 81 Serum (n=5) 78-90 81 EDTA Plasma (n=5) 97-105 101 EDTA Plasma (n=5) 97-105 101 Heparin Plasma (n=5) 80-103 99 Heparin Plasma (n=5) 80-103 99
Sensitivity
1.25 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Ctsg Microplate 96 Wells (12 x 8 Well Strips) Ctsg Lyophilized Standard 2 100X Biotinylated Ctsg Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Ctsg Microplate 96 Wells (12 x 8 Well Strips) Anti-Ctsg Microplate 96 Wells (12 x 8 Well Strips) Ctsg Lyophilized Standard 2 Ctsg Lyophilized Standard 2 100X Biotinylated Ctsg Detector Antibody 120 uL 100X Biotinylated Ctsg Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Cathepsin G (CTSG) . No significant cross-reactivity or interference between Cathepsin G (CTSG) and analogues was observed.
NCBI Gene Symbol
Ctsg
Protein Name
Cathepsin G
Gene Name URL
Ctsg
Nucleotide Accession Number
NM_007800.2

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