BCA Protein Colorimetric Detection Kit

• Reproducibility:

  _x005F Sample Protein Conc. (ug/mL) %CV _x005F 1 584.9 3.5 _x005F 2 285.5 6.9 _x005F 3 118.4 5.5 _x005F Sample Protein Conc. (ug/mL) %CV Sample Protein Conc. (ug/mL) %CV 1 584.9 3.5 1 584.9 3.5 2 285.5 6.9 2 285.5 6.9 3 118.4 5.5 3 118.4 5.5 _x005F Sample Protein Conc. (ug/mL) %CV _x005F 1 631.1 3.8 _x005F 2 297.7 6.3 _x005F 3 119.6 10.4 _x005F Sample Protein Conc. (ug/mL) %CV Sample Protein Conc. (ug/mL) %CV 1 631.1 3.8 1 631.1 3.8 2 297.7 6.3 2 297.7 6.3 3 119.6 10.4 3 119.6 10.4

• Type:

  Application Kit

• Detection Method:

  Colorimetric

• Assay Principle:

  The BCA Protein Assay Kit is designed to quantitatively measure total protein content in a variety of_x005F samples. The assay measures all types of proteins from all species. Please read the complete kit insert before_x005F performing this assay. A bovine serum albumin (BSA) standard is provided to generate a standard curve for_x005F the assay and all samples should be read off of the standard curve. Samples are diluted in water and added_x005F to the wells. The BCA Color Solution is made by mixing the BCA Reagent with the BCA Enhancer. The BCA_x005F Color Solution is added to all wells and the plate incubated at 37C. Protein in the samples reacts with the BCA_x005F Color Reagent to generate a purple colored product which is read at 560 nm.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates and Cell Lysates

• Sensitivity:

  Regular: 6.65 ug/mL. High sensitivity: 1.68 ug/mL.

• Reconstitution:

  RT

• Components:

  _x005F Component Quantity _x005F Clear Half Area 96 Well Plates 2 Plates _x005F Bovine Serum Albumin Standard 200 uL _x005F BCA Reagent 16 mL _x005F BCA Enhancer 320 uL _x005F Plate Sealers 2 Each _x005F Component Quantity Component Quantity Clear Half Area 96 Well Plates 2 Plates Clear Half Area 96 Well Plates 2 Plates Bovine Serum Albumin Standard 200 uL Bovine Serum Albumin Standard 200 uL BCA Reagent 16 mL BCA Reagent 16 mL BCA Enhancer 320 uL BCA Enhancer 320 uL Plate Sealers 2 Each Plate Sealers 2 Each

• NCBI Gene Symbol:

  BCA

• Gene Name URL:

  BCA