Formaldehyde Detection Kit

• Reproducibility:

  Sample Formaldehyde Conc. (uM) %CV 1 9.70 7.3 2 38.3 4.2 3 76.0 3.4 4 162 3.7 Sample Formaldehyde Conc. (uM) %CV Sample Formaldehyde Conc. (uM) %CV 1 9.70 7.3 1 9.70 7.3 2 38.3 4.2 2 38.3 4.2 3 76.0 3.4 3 76.0 3.4 4 162 3.7 4 162 3.7 Sample Formaldehyde Conc. (uM) %CV 1 10.5 6.7 2 36.9 4.5 3 71.1 3.8 4 148.9 4.3 Sample Formaldehyde Conc. (uM) %CV Sample Formaldehyde Conc. (uM) %CV 1 10.5 6.7 1 10.5 6.7 2 36.9 4.5 2 36.9 4.5 3 71.1 3.8 3 71.1 3.8 4 148.9 4.3 4 148.9 4.3

• Gene Name:

  Formaldehyde

• Type:

  Application Kit

• Assay Principle:

  The DetectX® Formaldehyde Kit is designed to quantitatively measure formaldehyde present in tissue culture media and urine samples. Please read the complete kit insert before performing this assay. A formaldehyde standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a black microtiter plate. The fluorescent reaction is initiated with the DetectX® Formaldehyde reagent, which is pipetted into each well. After a short incubation the emission of the generated fluorescent signal is detected in a microtiter plate reader capable of measuring 510 nm fluorescence utilizing 450 nm excitation wavelength. The concentration of the formaldehyde in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most fluorescence plate readers.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Human Urine and Tissue Culture Media

• Sensitivity:

  0.715 uM

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Black Half Area 96 Well Plate 2 plates Formaldehyde Standard 500 uL DetectX® Formaldehyde Reagent 5 mL Plate Sealers 2 each Component Quantity Component Quantity Black Half Area 96 Well Plate 2 plates Black Half Area 96 Well Plate 2 plates Formaldehyde Standard 500 uL Formaldehyde Standard 500 uL DetectX® Formaldehyde Reagent 5 mL DetectX® Formaldehyde Reagent 5 mL Plate Sealers 2 each Plate Sealers 2 each