Glucose Fluorescent Detection Kit

• Reproducibility:

  Sample Glucose Conc. (mg/dL) %CV 1 5.03 5.3 2 1.90 2.5 3 0.64 1.9 Sample Glucose Conc. (mg/dL) %CV Sample Glucose Conc. (mg/dL) %CV 1 5.03 5.3 1 5.03 5.3 2 1.90 2.5 2 1.90 2.5 3 0.64 1.9 3 0.64 1.9 Sample Glucose Conc. (mg/dL) %CV 1 4.51 13.3 2 1.86 6.2 3 0.65 8.3 Sample Glucose Conc. (mg/dL) %CV Sample Glucose Conc. (mg/dL) %CV 1 4.51 13.3 1 4.51 13.3 2 1.86 6.2 2 1.86 6.2 3 0.65 8.3 3 0.65 8.3

• Gene Name:

  Glucose

• Type:

  Application Kit

• Detection Method:

  Fluorometric

• Assay Principle:

  The DetectX® Glucose Fluorescent Detection Kit is designed to quantitatively measure glucose in a variety of samples. Please read the complete kit insert before performing this assay. A ß-D-glucose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Substrate and horseradish peroxidase and the reaction initiated by addition of glucose oxidase. The reaction is incubated at room temperature for 30 minutes. The glucose oxidase reacts with glucose to produce hydrogen peroxide which, in the presence of HRP, converts the substrate into a fluorescent product. The fluorescent product is read at 590 nm with excitation at 570 nm. Increasing levels of glucose cause a linear increase in fluorescence.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Urine, Buffers and TCM

• Sensitivity:

  Sensitivity was determined as 8.24 ug/dL. Equivalent to 0.00824 mg/dL.

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Black 96 well Half Area Plates 2 Plates Glucose Standard 90 uL Assay Buffer 50 mL Substrate 5 mL Horseradish Peroxidase Concentrate 60 uL Glucose Oxidase Concentrate 600 uL Component Quantity Component Quantity Black 96 well Half Area Plates 2 Plates Black 96 well Half Area Plates 2 Plates Glucose Standard 90 uL Glucose Standard 90 uL Assay Buffer 50 mL Assay Buffer 50 mL Substrate 5 mL Substrate 5 mL Horseradish Peroxidase Concentrate 60 uL Horseradish Peroxidase Concentrate 60 uL Glucose Oxidase Concentrate 600 uL Glucose Oxidase Concentrate 600 uL