Porcine IgG ELISpotBASIC kit (HRP)

Categories

ELISpot Kits
Description

Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 (included).
Enzyme Conjugate

NA
Format

without plates
Kit Detection Type

Streptavidin-HRP
Application

ELISpot
Reactivities

pig
Analytes

IgG
Contents

Capture mAb (MT421),Biotinylated detection mAb (MT424),Streptavidin-HRP,R848
Datasheet
Product Header

Basic ELISpot for enumeration of IgG secreting B cells
GeneID

NA
Shelf Life

18 months
Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Conjugation

HRP Conjugated
About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Gene target

ELISpotBASIC kit
Short name

Porcine IgG ELISpotBASIC kit ( )
Technique

IgG, IgGs
Isotype

IgG, IgG
Label

HRP
Species

Porcine
Alternative name

swine Immunoglobulin G ELISpotBASIC reagent (horseradish peroxidase)
Alternative technique

kits, immunoglobulins
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Enzyme-Linked Immunospot Assay
Concept

Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data