Bovine IgG ELISpotBASIC kit (ALP)

Categories

ELISpot Kits
Description

Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 (included).
Enzyme Conjugate

NA
Format

without plates
Kit Detection Type

Streptavidin-ALP
Application

ELISpot
Reactivities

cow
Analytes

IgG
Contents

Capture mAb (MT134),Biotinylated detection mAb (MT391),Streptavidin-ALP,R848
Datasheet
Product Header

Basic ELISpot for enumeration of IgG secreting B cells
GeneID

NA
Shelf Life

18 months
About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Gene target

ELISpotBASIC kit ALP
Gene symbol

PDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
Short name

IgG ELISpotBASIC kit (ALP)
Technique

IgG, IgGs
Isotype

IgG, IgG
Species

Bovine, Bos Taurus genes are available form the Bovine Genome database.
Alternative name

Bovine Immunoglobulin G ELISpotBASIC reagent (ALP)
Alternative technique

kits, immunoglobulins
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:11092
Gene

SLPI
Long gene name

secretory leukocyte peptidase inhibitor
Synonyms gene name
- secretory leukocyte protease inhibitor (antileukoproteinase)
Synonyms
- HUSI-I
- ALK1
- ALP
- BLPI
- HUSI
- WAP4
- WFDC4
Synonyms name
- antileukoproteinase
GenBank acession
- X04502
Locus

20q13.12
Discovery year

1998-04-24
Entrez gene record

6590
Pubmed identfication
- 3640338
- 12206714
Classification
- WAP four-disulfide core domain containing
VEGA ID

OTTHUMG00000033075

Identity

HGNC:10626
Gene

CCL27
Long gene name

C-C motif chemokine ligand 27
Synonyms gene
- SCYA27
Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 27
- chemokine (C-C motif) ligand 27
Synonyms
- ALP
- ILC
- CTACK
- skinkine
- ESkine
- PESKY
- CTAK
Synonyms name
GenBank acession
- AJ243542
Locus

9p13.3
Discovery year

1999-07-30
Entrez gene record

10850
Pubmed identfication
- 10556532
- 10588729
RefSeq identity
- NM_006664
Classification
- Chemokine ligands
VEGA ID

OTTHUMG00000019834

Name

Enzyme-Linked Immunospot Assay
Concept

Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data