Mouse IgE ELISpotBASIC kit (HRP)
-
Catalog number
3815-2H
-
Price
Please ask
-
Size
for 4 plates
-
-
Categories
ELISpot Kits
-
Description
IgE secretion can be induced by pre-activation of cells with the included anti-CD40 mAb and IL-4. Please note that stimulation with anti-CD40/IL-4 induces switching to IgE and IgG1 and results with stimulated cells may therefore not be representative of the situation in vivo.
-
Enzyme Conjugate
NA
-
Format
without plates
-
Kit Detection Type
Streptavidin-HRP
-
Application
ELISpot
-
Reactivities
mouse
-
Analytes
IgE
-
Contents
Capture mAb (MT56E),Biotinylated detection mAb (MT44E),Streptavidin-HRP,Anti-CD40 mAb,Recombinant mouse IL-4
-
-
Product Header
Basic ELISpot for enumeration of IgE secreting B cells
-
GeneID
NA
-
Shelf Life
18 months
-
Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
-
Conjugation
HRP Conjugated
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene target
-
Short name
Mouse IgE ELISpotBASIC kit ( )
-
Technique
Mouse, IgE, IgEs, mouses
-
Host
mouse
-
Isotype
IgE, IgE
-
Label
HRP
-
Species
Mouse, Mouses
-
Alternative name
Mouse immunoglobuline E ELISpotBASIC reagent (horseradish peroxidase)
-
Alternative technique
murine, kits, immunoglobulin-e
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
MeSH Data
-
Name
-
Concept
Scope note:
A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
-
Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products