Mouse IgM ELISpotBASIC kit (HRP)
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Catalog number3885-2H
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Price:
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Sizefor 4 plates
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CategoriesELISpot Kits
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DescriptionAntigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).
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Enzyme ConjugateNA
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Formatwithout plates
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Kit Detection TypeStreptavidin-HRP
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ApplicationELISpot
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Reactivitiesmouse
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AnalytesIgM
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ContentsCapture mAb (MT6A3),Biotinylated detection mAb (MT9A2),Streptavidin-HRP,R848,Recombinant mouse IL-2
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Product HeaderBasic ELISpot for enumeration of IgM secreting B cell
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GeneIDNA
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Shelf Life18 months
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PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationHRP Conjugated
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse IgM ELISpotBASIC kit ( )
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TechniqueMouse, IgM, IgMs, mouses
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Hostmouse
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IsotypeIgM, IgM
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LabelHRP
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SpeciesMouse, Mouses
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Alternative nameMouse Immunoglobulin M ELISpotBASIC reagent (horseradish peroxidase)
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Alternative techniquemurine, kits, immunoglobulin-m
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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ConceptScope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
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Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data