Mouse IgM ELISpotBASIC kit (HRP)

• Catalog number
  3885-2H
• Price
  Please ask
• Size
  for 4 plates

• Categories
  ELISpot Kits
• Description
  Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).
• Enzyme Conjugate
  NA
• Format
  without plates
• Kit Detection Type
  Streptavidin-HRP
• Application
  ELISpot
• Reactivities
  mouse
• Analytes
  IgM
• Contents
  Capture mAb (MT6A3),Biotinylated detection mAb (MT9A2),Streptavidin-HRP,R848,Recombinant mouse IL-2
• Datasheet
• Product Header
  Basic ELISpot for enumeration of IgM secreting B cell
• GeneID
  NA
• Shelf Life
  18 months
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Conjugation
  HRP Conjugated
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  ELISpotBASIC   kit  
• Short name
  Mouse IgM ELISpotBASIC kit ( )
• Technique
  Mouse, IgM, IgMs, mouses
• Host
  mouse
• Isotype
  IgM, IgM
• Label
  HRP
• Species
  Mouse, Mouses
• Alternative name
  Mouse Immunoglobulin M ELISpotBASIC reagent (horseradish peroxidase)
• Alternative technique
  murine, kits, immunoglobulin-m
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Enzyme-Linked Immunospot Assay
• Concept
  Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
• Tree numbers
  • E01.370.225.500.508
  • E05.200.500.508
  • E05.242.551
  • E05.478.566.350.170.500
  • E05.478.566.380.360.500
  • E05.478.583.400.170.500
  • E05.601.470.350.170.500
  • E05.601.470.380.360.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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