Human IgG ELISpotBASIC kit (ALP)

• Catalog number
  3850-2A
• Price
  Please ask
• Size
  for 4 plates

• Categories
  ELISpot Kits
• Description
  Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
• Enzyme Conjugate
  ALP
• Format
  without plates
• Kit Detection Type
  Streptavidin-ALP
• Application
  ELISpot
• Reactivities
  human, non-human primates
• Analytes
  IgG
• Contents
  Capture mAbs (MT91/145),Biotinylated detection mAbs (MT78/145),Streptavidin-ALP,R848,Recombinant human IL-2
• Datasheet
• Product Header
  Basic ELISpot for enumeration of IgG secreting B cells
• GeneID
  The monoclonal antibodies cross-react with IgG from non-human primates. R848 and human IL-2 can be used for stimulation of B cells from non-human primates
• Shelf Life
  18 months
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  ELISpotBASIC   kit   ALP  
• Gene symbol
  PDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
• Short name
  IgG ELISpotBASIC kit (ALP)
• Technique
  IgG, IgGs
• Isotype
  IgG, IgG
• Species
  Human, Humans
• Alternative name
  H. sapiens Immunoglobulin G ELISpotBASIC reagent (ALP)
• Alternative technique
  kits, immunoglobulins
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:20767
• Gene
  PDLIM3
• Long gene name
  PDZ and LIM domain 3
• Synonyms
  • ALP
• GenBank acession
  • AF002280
• Locus
  4q35.1
• Discovery year
  2004-02-06
• Entrez gene record
  27295
• Pubmed identfication
  • 10063829
  • 8828038
• RefSeq identity
  • NM_014476
• Classification
  • PDZ domain containing
  • LIM domain containing
• VEGA ID
  OTTHUMG00000160412
• Locus Specific Databases
  • LRG_752

Gene info

• Identity
  HGNC:16448
• Gene
  ASRGL1
• Long gene name
  asparaginase and isoaspartyl peptidase 1
• Synonyms gene name
  • asparaginase like 1
• Synonyms
  • FLJ22316
  • ALP1
  • ALP
• Synonyms name
  • asparaginase-like 1 protein
• Locus
  11q12.3
• Discovery year
  2001-08-29
• Entrez gene record
  80150
• Pubmed identfication
  • 11984834
  • 28671681
  • 27106100
• RefSeq identity
  • NM_001083926
• VEGA ID
  OTTHUMG00000167513

Gene info

• Identity
  HGNC:29063
• Gene
  ATRNL1
• Long gene name
  attractin like 1
• Synonyms gene name
  • attractin-like 1
• Synonyms
  • KIAA0534
  • FLJ45344
  • ALP
• GenBank acession
  • AB011106
• Locus
  10q25.3
• Discovery year
  2004-03-04
• Entrez gene record
  26033
• Pubmed identfication
  • 9628581
• RefSeq identity
  • NM_207303
• Classification
  • C-type lectin domain containing
• VEGA ID
  OTTHUMG00000019096

Gene info

• Identity
  HGNC:11092
• Gene
  SLPI
• Long gene name
  secretory leukocyte peptidase inhibitor
• Synonyms gene name
  • secretory leukocyte protease inhibitor (antileukoproteinase)
• Synonyms
  • HUSI-I
  • ALK1
  • ALP
  • BLPI
  • HUSI
  • WAP4
  • WFDC4
• Synonyms name
  • antileukoproteinase
• GenBank acession
  • X04502
• Locus
  20q13.12
• Discovery year
  1998-04-24
• Entrez gene record
  6590
• Pubmed identfication
  • 3640338
  • 12206714
• Classification
  • WAP four-disulfide core domain containing
• VEGA ID
  OTTHUMG00000033075

Gene info

• Identity
  HGNC:10626
• Gene
  CCL27
• Long gene name
  C-C motif chemokine ligand 27
• Synonyms gene
  • SCYA27
• Synonyms gene name
  • small inducible cytokine subfamily A (Cys-Cys), member 27
  • chemokine (C-C motif) ligand 27
• Synonyms
  • ALP
  • ILC
  • CTACK
  • skinkine
  • ESkine
  • PESKY
  • CTAK
• Synonyms name
  • CC chemokine ILC
  • IL-11 Ralpha-locus chemokine
  • cutaneous T-cell attracting chemokine
• GenBank acession
  • AJ243542
• Locus
  9p13.3
• Discovery year
  1999-07-30
• Entrez gene record
  10850
• Pubmed identfication
  • 10556532
  • 10588729
• RefSeq identity
  • NM_006664
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000019834

MeSH Data

• Name
  Enzyme-Linked Immunospot Assay
• Concept
  Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
• Tree numbers
  • E01.370.225.500.508
  • E05.200.500.508
  • E05.242.551
  • E05.478.566.350.170.500
  • E05.478.566.380.360.500
  • E05.478.583.400.170.500
  • E05.601.470.350.170.500
  • E05.601.470.380.360.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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