Mouse MHC Class II antibody (FITC)
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Catalog number
61R-M110aFT
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Price
Please ask
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Size
120 tests
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Applications
FC, WB
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Product Type
Primary Antibodies
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Product Subtype
Conjugated Monoclonal Antibodies
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Research Area
Immunology
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Immunogen
MHC class II antibody (FITC) was raised in mouse using purified class II from JY cell line as the immunogen.
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Specificity
Human
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Cross Reactivity
To be determined by end-user
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Clone
TDR31.1
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Concentration
0.25 mg/ml
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Form Buffer
50mM Na3PO4, pH 7.5, with 100mM KCL, 150mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
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Storage
Store at 4 deg C. DO NOT FREEZE.
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Shipping Info
Blue Ice
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CD74, BTNL2, RFX1
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Short name
Mouse MHC Class II antibody (FITC)
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Technique
Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, mouses
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Host
Mouse
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Isotype
IgG1
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Mouse monoclonal MHC Class II antibody (FITC)
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Alternative technique
antibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene name
CD74 molecule
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Synonyms gene
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Synonyms gene name
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
- CD74 molecule, major histocompatibility complex, class II invariant chain
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Synonyms
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Synonyms name
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
butyrophilin like 2
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Synonyms gene name
- butyrophilin-like 2 (MHC class II associated)
- butyrophilin-like 2
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Butyrophilins
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
regulatory factor X1
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Synonyms gene name
- regulatory factor X, 1 (influences HLA class II expression)
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Synonyms
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Synonyms name
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Locus
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Discovery year
1991-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Regulatory factor X family
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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