Goat Biotin antibody (FITC)

• Catalog number
  60C-CF0301
• Price
  Please ask
• Size
  20 mg

• Applications
  IF, FC
• Product Type
  Primary Antibodies
• Product Subtype
  Conjugated Polyclonal Antibodies
• Research Area
  Miscellaneous
• Immunogen
  Biotin antibody (FITC) was raised in goat using biotin conjugated KLH as the immunogen.
• Specificity
  NA
• Cross Reactivity
  To be determined by end-user
• Clone
  NA
• Concentration
  NA
• Form Buffer
  Liquid in 0.01M Sodium Phosphate, 0.14M NaCl, pH 7.4 with 10mg/ml BSA (IgG and Protease free) and 0.01% Sodium Azide.
• Storage
  Store at 4 deg C short term, -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
• Shipping Info
  Blue Ice
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Biotinylated, Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus
• French translation
  anticorps

• Short name
  Goat antibody (FITC)
• Technique
  Antibody, FITC, Goat, antibodies against human proteins, antibodies for, Fluorescein, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  NA
• Label
  Biotin
• Species
  Goats
• Alternative name
  Goat polyclonal Biotin antibody (FITC) conjugated
• Alternative technique
  antibodies, caprine, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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