Goat FITC antibody (HRP)
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Catalog number60R-FG002hrp
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PricePlease ask
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Size1 mg
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ApplicationsELISA, IHC, WB
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Product TypePrimary Antibodies
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Product SubtypeConjugated Polyclonal Antibodies
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Research AreaMiscellaneous
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ImmunogenFITC antibody (HRP) was raised in goat using fluorescein conjugated to goat IgG as the immunogen.
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SpecificityNA
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Cross ReactivityTo be determined by end-user
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CloneNA
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ConcentrationNA
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Form BufferLyophilized from 0.02M K2PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.
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StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
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Shipping InfoBlue Ice
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationAnti-FITC Antibody, HRP Conjugated
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Latin nameCapra aegagrus hircus
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French translationanticorps
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Short nameGoat FITC antibody ( )
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TechniqueAntibody, FITC, Goat, antibodies against human proteins, antibodies for, Fluorescein, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeNA
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LabelFITC
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SpeciesGoats
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Alternative nameGoat polyclonal FITC antibody (HRP) conjugated
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Alternative techniqueantibodies, caprine, fluorescine
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data