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Category
Primary Antibody
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Antibody Subtype
Conjugated Polyclonal Antibodies
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Area of research
Miscellaneous
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Type of Immunogen
Biotin antibody (FITC) was raised in goat using biotin conjugated KLH ..
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Raised in
Goat
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
FITC
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Method of Purification
Biotin antibody (FITC) was purified by ion exchange chromatography followed by dialysis.
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Form Buffer
Liquid in 0.01M Sodium Phosphate, 0.14M NaCl, pH 7.4 with 10mg/ml BSA (IgG and Protease free) and 0.01% NaN3.
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Storage
Store at 4 deg C short term, -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
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Shipping conditions
Blue Ice
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Tested for
IF; FC
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Usage Recommendations
FC: 1:2,000-1:10,000, IF: 1:500-1:2,500
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Biotinylated, Anti-FITC Antibody
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French translation
anticorps
