TIP2;1, tonoplast intrinistic protein 2-1
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Catalog numberAS09 509
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PricePlease ask
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Size100 µl
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Available ordering formatInquire
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ImmunogenKLH-cionjugated peptide derived from Oryza sativa TIP2-1 Q7XA61
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationSerum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionstore at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applicationsELISA (ELISA), western blot (WB)
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Connected productscollection of antibodies to tonoplast proteins AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 494 | anti-TIP2;1 (delta) | Delta-VM23AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1AS09 510 | anti-TIP2;2 | tonoplast intrinistic protein 2-2AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 8000 (ELISA), 1: 2000 with standard ECL (WB), immunolocalization (IL)
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Molecular weight expected аpparent24.8 | 20-24 kDa
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Verified reactivityOryza sativa, Populus trichocarpa x deltoides, clone H11-11
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Possible reactivityGladiolus hybrid, Hordeum vulgare, Triticum aestivum, Zea mays
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No reactivityArabidopsis thaliana
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Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
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ReferencesSakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
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Scientific contextTIP2-1 belongs to aquaporin protein family and facilitates transport of water and small neutral solutes across tonoplast membrane.
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Notes0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Vacuolar membrane protocol is available here.
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Protein numberQ7XA61
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TAIR numberRefer to NCBI
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Gene target
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Gene symbolIGHVIII-2-1, MIR1-2, TRUND-NNN9-1, SLC2A4RG, TRH-GTG2-1, MIR511, TRN-ATT1-2, TRG-CCC2-1, TRS-AGA2-1, TRC-GCA2-2
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Short nameTIP2 1, tonoplast intrinistic protein 2-1
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Alternative nameTIP2;1, tonoplast intrinistic protein 2-1
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-2-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-2-1
- immunoglobulin heavy variable (III)-2-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 9-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 9-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameSLC2A4 regulator
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-His (GTG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 11 (anticodon GUG)
- transfer RNA-His (GTG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene namemicroRNA 511
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Synonyms gene
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Synonyms gene name
- microRNA 511-2
- microRNA 511-1
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene nametRNA-Asn (ATT) 1-2
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Synonyms gene name
- transfer RNA-Asn (ATT) 1-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2014-07-07
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Entrez gene record
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Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gly (anticodon CCC) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA glycine 22 (anticodon CCC)
- transfer RNA-Gly (CCC) 2-2
- transfer RNA-Gly (CCC) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Ser (anticodon AGA) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA serine 25 (anticodon AGA)
- transfer RNA-Ser (AGA) 2-2
- transfer RNA-Ser (AGA) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Cys (anticodon GCA) 2-2
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Synonyms gene
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Synonyms gene name
- transfer RNA cysteine 5 (anticodon GCA)
- transfer RNA-Cys (GCA) 2-1
- transfer RNA-Cys (GCA) 2-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs