PIP1;3, aquaporin, plasma membrane intrinistic protein 1-3
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Catalog numberAS09 504
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PricePlease ask
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Size100 µl
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Available ordering formatInquire
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Immunogensynthetic peptide derived from N-terminal part of Oryza sativa PIP1-3 Q9SXF8
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationSerum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionstore at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applicationsELISA (ELISA), western blot (WB)
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Connected productscollection of antibodies to plasma membrane proteins AS09 505 | anti-PIP1s | aquaporinAS09 506 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5Secondary antibodies
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Recommended dilutions for use1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
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Molecular weight expected аpparent30.7 | 26-29 kDa (Oryza sativa)
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Verified reactivityOryza sativa, Zea mays
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Possible reactivitymonocots including: Hordeum vulgare, Stipa sp. Triticum aestivum,
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
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ReferencesSakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
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Scientific contextPIP1;3 is a plasma membrane aquaporine which facilitates trasport of water across cell membrane. Alternative names: aquaporin PIP 1-3, water channel protein RWC3, RWC-3
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Notes0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plasma membrane can be found here.
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Protein numberQ9SXF8
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TAIR numberRefer to NCBI
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DescriptionAssociated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
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Gene target
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Gene symbolACD, YWHAZ, TRR-CCG1-3, TRR-ACG1-3, TRW-CCA3-3, TRF-GAA1-3, MIR3130-1, TRP-CGG1-3, TRI-GAT1-3, TRH-GTG1-3
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Short namePIP1 3, aquaporin, plasma intrinistic protein 1-3
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Alternative namePIP1;3, aquaporin, plasma membrane intrinistic protein 1-3
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Gene info
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Identity
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Gene
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Long gene nameACD shelterin complex subunit and telomerase recruitment factor
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Synonyms gene name
- adrenocortical dysplasia homolog (mouse)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-01-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Shelterin complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
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Synonyms gene
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Arg (anticodon CCG) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA arginine 19 (anticodon CCG)
- transfer RNA-Arg (CCG) 1-1
- transfer RNA-Arg (CCG) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Arg (anticodon ACG) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA arginine 21 (anticodon ACG)
- transfer RNA-Arg (ACG) 1-1
- transfer RNA-Arg (ACG) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Trp (anticodon CCA) 3-3
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Synonyms gene
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Synonyms gene name
- transfer RNA tryptophan 3 (anticodon CCA)
- transfer RNA-Trp (CCA) 3-1
- transfer RNA-Trp (CCA) 3-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 11 (anticodon GAA)
- transfer RNA-Phe (GAA) 1-1
- transfer RNA-Phe (GAA) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene namemicroRNA 3130-1
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Synonyms gene
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Synonyms gene name
- microRNA 3130-3
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Synonyms
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Locus
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Discovery year2010-04-30
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Pro (anticodon CGG) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA proline 8 (anticodon CGG)
- transfer RNA-Pro (CGG) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon GAT) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 26 (anticodon GAU)
- transfer RNA-Ile (GAT) 1-3
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Synonyms
-
GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 9 (anticodon GUG)
- transfer RNA-His (GTG) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs